Liquid dish wash solution – can it be an alternative in future for the expensive and hazardous xylene in hematoxylin and eosin staining of paraffin sections

Swaroop Raj B.V, Divya C, Harendra Kumar ML

Abstract


Introduction: Xylene is the most commonly used clearing agent as it has excellent deparaffinising and clearing properties. Commercial xylene is a clear, colourless liquid that has a sweet and aromatic odour. However, xylene is not only expensive but also has detrimental effect on human health such as hepatitis, chemical pneumonitis, depression, anaemia, etc. Hence in this study we are replacing xylene with commercially available liquid dish wash solution(LDW) which is not only cheap but also non biohazardous. Aims: To assess the efficacy of LDW with xylene as a deparaffinising and clearing agent in routine hematoxylin and eosin staining (H&E). Materials and Methods: Blocks from 20 consecutively submitted samples at RL Jalappa Hospital between Jan – June 2015 each of breast, Cervix, Lymphnode, Fat, Salivary Gland, Muscle and Alimentary Tract was taken and two sections of 4-micron thickness were cut and deparaffinised using Liquid Dish Wash and Xylene respectively followed by H & E Staining. The 280 sections of the 140 blocks were evaluated and scored by two pathologists independently for nuclear staining, cytoplasmic staining, uniformity, clarity and crispness. Results: In the samples evaluated LDW seems to be on par with xylene cleared sections in terms of nuclear and cytoplasmic detail, clarity and crispness. However Xylene cleared sections showed slightly better uniform staining. However both sections were equally good for reporting. Conclusion: Liquid dish wash solution is an excellent alternative to xylene in H&E Staining. However more extensive studies need to be done on other types of tissues for validation

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References


Kandyala R, Raghavendra SP, Rajasekharan ST. Xylene: An overview of its health hazards and preventive measures. J Oral Maxillofac Pathol. 2010 Jan; 14 (1):1-5. doi: 10.4103/0973-029X.64299.

Ramulu S, Koneru A, Ravikumar S, Sharma P, Ramesh D, Patil R. Liquid dish washing soap: an excellent substitute for xylene and alcohol in hemato-xylin and eosin staining procedure. J Orofac Sci 2012; 4 (1): 37‑42.doi: 10.4103/0975-8844.99890.

Falkeholm L, Grant CA, Magnusson A, Möller E. Xylene-free method for histological preparation:a multi-centre evaluation.LabInvest.2001Sep;81(9):1213-21.

Ankle MR, Joshi PS. A study to evaluate the efficacy of xylene free hematoxylin and eosin staining procedure as compared to the conventional hematoxylin and eosin staining: An experimental study.JOMFP 2011;15(2): 161-167. doi: 10.4103/0973-029X.84482.

Pinki Pandey, Alok Dixit, Aparna Tanwar, Anuradha Sharma, Sanjeev Mittal. A comparative study to evaluate liquid dish washing soap as an alternative to xylene and alcohol in deparaffinization and hemato-xylin and eosin staining. J Lab Physicians 2014; 6 (2):84-90. doi: 10.4103/0974-2727.141504.

Anuradha Ananthaneni, Srilekha Namala, Vijay Srinivas Guduru, et al., Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study. Scientifica. 2014; 2014: 11.doi.org/10.1155/ 2014 /707310.

Buesa RJ, Peshkov MV. Histology without xylene. Ann Diagn Pathol. 2009 Aug;13(4): 246-56. doi: 10. 1016/j. anndiagpath. 2008.12.005. Epub 2009 Feb 5.

Hamil JR, Spencer JM, Morgan MB. Histotechnique and staining troubleshooting. On Atlas of Mohs and Frozen Section Cutaneous Pathology. Ch. 20. New York: Springer Link; 2009.

Chen CY, He T, Mao XL, Friis TE, Qin RH, Jian YT. A novel xylene substitute for histotechnology and histochemistry. Biotech Histochem. 2010 Aug; 85 (4): 231-40.doi: 10.3109/10520290903235445.

Miller M, Miller D, Driscoll PE. Biodegradable, effective substitute for xylene in the ehrlich indole procedure. J Clin Microbiol 1994;32:2028-30.

Foti C, Zambonin CG, Conserva A, et al. Occupational contact dermatitis to a limonene-based in a histopathology technician. Contact Dermat 2007;56 (2): 109-12.

Tarbet F, Anderson J. Toxin reduction in Histology. A look at advantages, disadvantages of xylene substitutes. Advance AdmLab 2005;14(4):42.


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